An isothermal in-vitro nucleotide amplification process. The process involves the concomitant action of a RNA-DIRECTED DNA POLYMERASE, a ribonuclease (RIBONUCLEASES), and DNA-DIRECTED RNA POLYMERASES to synthesize large quantities of sequence-specific RNA and DNA molecules.

Synonyms: Nucleic Acid Sequence Based Amplification, Analyses, NASBA, NASBA Analyses, Self-Sustained Sequence Replication, Replications, Self-Sustained Sequence, Sequence Replication, Self-Sustained, Self Sustained Sequence Replication, Replication, Self-Sustained Sequence, Analysis, NASBA, Self-Sustained Sequence Replications, Sequence Replications, Self-Sustained, NASBA, NASBA Analysis, Nucleic Acid Sequence-Based Amplification